FIG. 8.

Southern blot analysis of sgs1Δ strains that sustained a Ty1HIS3 transposition event. (A) Three schematics showing the size of the HIS3-containing HpaI fragment from a monomeric Ty1HIS3 element within chromosomal DNA or a one-LTR or two-LTR Ty1HIS3:Ty1 multimer. (B) Results of hybridization of a ³²P-labeled HIS3 riboprobe to HpaI-digested genomic DNA from 13 sgs1Δ Ty1his3AI-236 strains harboring a Ty1HIS3 element. DNA samples containing a one-LTR or two-LTR Ty1HIS3:Ty1 multimer, as demonstrated by PCR (Fig. 7), are labeled accordingly. The sizes of the HpaI bands derived from Ty1his3AI-236 (6.1 kb) and from one-LTR (6.4 kb) and two-LTR (6.7 kb) Ty1HIS3:Ty1 multimers are indicated. The sizes of the bands derived from monomeric Ty1HIS3 elements, which are expected to be >5.9 kb, are not indicated.