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Nuclear export of PI-9 is blocked by LMB. COS-1 cells transfected with the expression vectors encoding PI-9 or RevMAPKK/GFP were exposed to 4 ng of LMB/ml for the indicated times. Cells were prepared for visualization (A) and quantitation (B) of nuclear/cytoplasmic ratios (Fn/c) or nucleolar/cytoplasmic ratios (Fnu/c by CLSM as described in Materials and Methods). Results are the mean ± SEM, with n being the number of cells analyzed.
