FIG. 4.

Effects of P_i and ADP on PNPase activity. Purified PNPase (150 ng) was incubated with [³²P]RNA representing the psbA gene (marked by an arrowhead) for 45 min with the addition of P_i and ADP in the concentrations shown. The first lane in each panel (marked C) represents the input RNA. In panel C, the RNA was incubated with purified PNPase and 1 mM ADP. The RNA was purified and analyzed at the time points indicated at the bottom. Schematic representations of the RNA molecules are presented on the right and left.