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. 2001 Aug;21(16):5408–5416. doi: 10.1128/MCB.21.16.5408-5416.2001

FIG. 7.

FIG. 7

A stem-loop structure inhibits the polymerization activities of both lysed chloroplasts and purified PNPase. A 106-nucleotide [32P]RNA fragment representing the malE-malF intergenic region of E. coli with or without the addition of six cytosine residues, as shown schematically at the bottom, was incubated with lysed chloroplasts or purified PNPase with the addition of ADP (0.5 mM), ATP (1 mM), or GTP (1 mM) as indicated. Lane (-), input RNA. Following an incubation of 5 min for the lysed chloroplasts and 25 min for the purified PNPase, RNA was purified and analyzed.