FIG. 6.

Effects of overexpression of Dok-1 mutants on cell migration on ECM proteins. The indicated cell lines were seeded onto porous membranes that had been both coated with fibronectin (A), vitronectin (B), or type 4 collagen (C) and placed in Boyden multiwell chambers. After incubation at 37°C for 3 h, cells that had migrated were stained with Giemsa solution. The number of migrated cells was counted and expressed as a percentage of the value for control cells transfected with the empty vector (Cont). Data are means ± SE of triplicate determinations from three independent experiments.