Figure 4.

KLHL38 is the adaptor that targets BECN1 for ubiquitination and degradation. (A) The expression of CUL3 adaptors in breast cancer tissue compared to normal tissue. (B) KLHL38 affects the expression of BECN1. MDA-MB-231 cells were transfected with indicated siRNAs, and the expression of BECN1 is determined by immunoblotting. (C) The effect of KLHL38 siRNAs on the transcription of BECN1 was determined by qRT-PCR. Data are represented as the mean ± SD. (D) CUL3 complex promotes the proteasomal degradation of BECN1. MDA-MB-231 cells were transfected with indicated plasmids, treated with MG132 for 6 h, and the expression of BECN1 is determined by immunoblotting. (E) KLHL38 interacts with BECN1. 293 t cells overexpressing KLHL38 and BECN1 were treated with 10 μM MG132 for 6 h, and the immunoprecipitation was performed. (F) Knockdown of KLHL38 affects BECN1 ubiquitination. 293 t cells were transfected with the indicated plasmids or siRNAs and the immunoprecipitation was performed