Figure 5.

CUL3 mediated tumorigenesis through BECN1 degradation. (A), (B) Hela or 231 cells were transfected with siRNAs targeting CUL3 for the indicated days. Cell viability was measured by MTT assay. (C) 231 cells are transfected with siRNA targeting KLHL38 for the indicated days. Cell viability is measured by MTT assay. (D-F,H), Cells were transfected with indicated siRNAs or plasmids and the clone formation assay was performed. Cells were fixed and stained by crystal violet. (G) 231 cells were transfected with plasmids encoding MYC-CUL3 for the indicated days. Cell viability was measured by MTT assay. (I) left, 231 cells stable transfected with shRNA targeting CUL3 were treated with the indicated amount of CQ. Cell viability was measured by MTT assay. right, 231 cells overexpressing CUL3 were treated with indicated amount of MLN4924 for 24 h. Cell viability was measured by MTT assay