Figure 2.

ETV4 stimulates the up-regulation of C2CD4D-AS1 a. Venn diagram demonstrated transcription factors with high binding scores in HumanTFDB overlapped with up-regulated transcription factors in LUAD. b-c. RT-qPCR tested the efficiency of ETV4 inhibition or overexpression, as well as the variations in C2CD4D-AS1 expression caused by ETV4 down-regulation or up-regulation. d. The binding sequence between ETV4 and C2CD4D-AS1 promoter was presented. e. The affinity of ETV4 and C2CD4D-AS1 promoter was examined through ChIP assay. f. In DNA pull down assay, the enrichment of ETV4 in Bio-C2CD4D-AS1 promoter was measured by western blot. g. The binding of ETV4 and C2CD4D-AS1 promoter was further confirmed by luciferase reporter assay. **P < 0.01, n.s.: no significance