Figure 5.

C2CD4D-AS1 accelerates LUAD cell proliferation, migration, invasion and suppresses cell apoptosis by modulating miR–3681–3p/NEK2 axis a. RT-qPCR analyzed NEK2 expression in response to NEK2 augment. b-c. Cell proliferation in sh-NC group, sh-C2CD4D-AS1#1 group, sh-C2CD4D-AS1#1+ miR-3681–3p inhibitor group and sh-C2CD4D-AS1#1+ pcDNA3.1/NEK2 group was detected by CCK8 and colony formation assays. d-e. Transwell assay was implemented for observation of cell migration and invasion in different groups. f. The apoptotic capacity of LUAD cells transfected with indicated plasmids was examined by TUNEL assay. **P < 0.01