Figure 4.

IGF1R levels are increased in SOD1^G93A iPSA. (A) Representative western blot and quantification of CTRL and G93A iPSA of p-AKT (Thr308) protein levels normalized to total AKT (n = 3), (B) IGF1R protein levels normalized to ACTB (n = 6). (C) IGF1R mRNA levels normalized to ACTB (n = 3). (D) CTRL and G93A iPSA were transduced with shSOD1 lentiviral particles (+) or negative control (-) for 48 h prior to the assays. Representative western blot and quantification of IGF1R protein levels normalized to ACTB (n = 4). P values <0.05 by unpaired Student’s t test (A-B) or by one-way ANOVA test with Sidak’s correction between the indicated groups (D) are shown. (E) Representative western blot of WT lines from healthy subjects (lane 1–5) and ALS iPSA (FUS, SOD1^AV4, Sporadic, C9orf72, SOD1^D90A in lane 2, 3, 4, 6, 7, respectively) and quantification of IGF1R protein levels normalized to ACTB