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Isoelectric points of the antibodies measured by cIEF and calculated from primary sequences
| Antibodies | Measured pI | Calculated pI |
||
|---|---|---|---|---|
| IgG | Fab | Fc | ||
| IgG1-A | 9.1 | 8.0 | 8.4 | 6.7 |
| IgG1EN-A | 9.0 | 7.9 | 8.4 | 6.4 |
| IgG2-A | 8.7 | 7.6 | 7.9 | 6.7 |
| IgG4PAA-A | 8.2 | 7.4 | 8.2 | 5.7 |
| IgG1-B | 7.1 | 6.7 | 6.4 | 6.7 |
| IgG1EN-B | 6.9 | 6.5 | 6.4 | 6.4 |
| IgG2-B | 6.4 | 6.2 | 5.7 | 6.7 |
| IgG4PAA-B | 6.1 | 5.9 | 6.1 | 5.7 |
| IgG1-C | 7.4 | 7.0 | 6.9 | 6.7 |
| IgG1EN-C | 7.2 | 6.8 | 6.9 | 6.4 |
| IgG2-C | 6.7 | 6.4 | 5.8 | 6.7 |
| IgG4PAA-C | 6.3 | 6.0 | 6.5 | 5.7 |
