Table 3.
HIC-HPLC retention time (minutes) |
Hydrophobicity interaction potential (HIP%) |
|||||
---|---|---|---|---|---|---|
IgG subclass | Fv A | Fv B | Fv C | Fv A | Fv B | Fv C |
IgG1 | 13.4 | 13.0 | 16.0 | 42.1 | 40.1 | 55.0 |
IgG1EN | 13.4 | 13.0 | 16.0 | 42.1 | 40.0 | 55.2 |
IgG2 | 13.5 | 13.0 | 16.1 | 42.3 | 40.1 | 55.7 |
IgG4PAA | 14.0 | 13.6 | 16.4 | 44.8 | 43.0 | 56.9 |