Figure 1.

LC3-II and SQSTM1/p62 expression and autophagic flux in H₂O₂-activated human platelets. (A) Representative scanning electron microscopy (SEM) images of platelets treated with or without 100 μM H₂O₂ for 60 min. Scale bars: 5 μm. (B and C) Washed platelets (1.2 × 10⁹/mL) treated with 50 and 100 μM H₂O₂ for 60 min in the presence or absence of Baf-A1 (200 nM) or 3-MA (3 mM). (D) Washed platelets treated with 100 μM H₂O₂ for the indicated time (0–120 min). LC3 and SQSTM1/p62 detected using specific antibodies through western blotting. Data (B-D) are presented as the means ± S.E.M. (n = 4). *P < 0.05, **P < 0.01, and ***P < 0.001, compared with the control group. ^#P < 0.05 and ^###P < 0.001 with the H₂O₂-treated group