Figure 3.

Regulatory effects of the AMPK-MTOR pathway on platelet autophagy. (A) Washed platelets treated with 100 μM H₂O₂ for the indicated time (0–60 min). (B-D) Washed platelets treated with 50 and 100 μM H₂O₂ for 60 min in the presence or absence of compound C (10 μM). (E) Washed platelets treated with 100 μM H₂O₂ for 60 min in the presence or absence of compound C (10 μM). Specific antibodies were used to detect p-PRKAA (Thr172), p-ULK (Ser317), p-MTOR (Ser2448), LC3, and SQSTM1/p62 by western blotting. Data are presented as the means ± S.E.M. (n = 4; SQSTM1, n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001, compared with the control platelets. ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 with the H₂O₂-treated group