Figure 3.

Deficiency of CREG1 enhanced mitophagy in the gastrocnemius of 9-month-old creg1;Ckm-Cre mice. (A and C) Western blotting (A) and quantification analysis showed the expression of PINK1 (B) and PRKN (C) in the mitochondria of the gastrocnemius of 3- and 9-month-old Creg1^fl/fl and creg1;Ckm-Cre mice. (D and F) Western blotting (D) and quantification analysis (E and F) showed the expression of LC3-II and SQSTM1 protein in the mitochondria of the gastrocnemius of 3- and 9-month-old Creg1^fl/fl and creg1;Ckm-Cre mice. (G) Confocal microscopy analysis of the colocalization of LC3B and TOMM20, yellow asterisks represented the colocalization of LC3B and TOMM20, scale bars: 50 μm. (H and I) Western blotting (H) and quantification analysis (I) showed the expression of SQSTM1 and LC3-II protein in the mitochondria of the gastrocnemius treated with colchicine (0.4 mg/kg·day). Data are shown as mean ± SEM, **p < 0.01, ^##p < 0.01