Figure 5.

Deficiency of CREG impaired the mitophagy in C2C12 cells. (A and B) Western blotting analysis to measure the expression of PINK1, PRKN, LC3-II and SQSTM1 in the mitochondria from the siScramble and siCreg1 cell groups. (C) Confocal images of colocalization of GFP-LC3B puncta and mitochondria (red-MitoTracker) in C2C12 cells transfected with the Creg1 siRNA during growth in CCCP (10 μM) for 1 h. Scale bar: 10 μm. (D) Quantification of colocalization of GFP-LC3B puncta and mitochondria. n = 3. (E and F) Western blotting (E) and quantification analysis (F) showed the expression of LC3-II and SQSTM1 protein in the mitochondria of the gastrocnemius treated with carbonyl cyanide m-chlorophenylhydrazone (CCCP, 10 μΜ, 1 h). Data are shown as mean ± SEM, **p < 0.01, ^##p < 0.01