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CREG1 directly interacted with the peptide fragment 401–573 aa of HSPD1. (A and C) Co-IP analysis to detect interaction between CREG1 and peptide fragments 1–200 aa (A), 201–400 aa (B), and 401–573 aa (C) of HSPD1. IP antibody: anti-FLAG, IB antibody: anti-CREG1. Input: CREG1. (D and E) Co-IP analysis to detect interaction between HSPD1 with different peptide fragments of CREG1: 1–100 aa (D) and 130–220 aa (E). IP antibody: anti-HIS, IB antibody: anti-HSPD1. Input: HSPD1
