Figure 3.

SOC alters intestinal microbiota composition. Mice were subjected to SOC or normal housing for 19 weeks with or without antibiotic treatment. Principal coordinates analysis of the unweighted UniFrac distance matrix of NH and SOC mice (a,c) and antibiotic-treated NH and SOC mice (b,d) at 0 and 19 weeks of treatment. LEfSe analysis for relative microbial abundance in SOC mice compared with NH mice at 19 weeks of treatment (e) and representation of select SOC-induced alteration of gut microbiota composition (f-j). Data are the means ± SEM, points represent individual mice (N = 20). Significance was determined using t-test or Anosim (*p ≤ 0,05; **p ≤ 0,01; ***p ≤ 0,001; n.s. indicates nonsignificant)