Figure 3.

Amelioration of non-alcoholic fatty liver disease by retinoic acid does not directly depend on the liver. (a) Representative photograph of the liver. (b) liver weight and liver/body weight. (c-f) Hepatic TG, TC, ALT, and AST levels. (g) H&E staining of the liver. (h) The effects of RA on OA- and PA-induced lipid accumulation in HepG2 cells. Intracellular lipid accumulation was measured by Oil Red O staining using an inverted microscope. The OD at 510 nm was measured. Data are shown as mean ± SEM. One-way ANOVA was performed to compare multiple groups, and differences (P < 0.05) have been labelled with different letters; same letters indicate no significance (P > 0.05)