Skip to main content
. 2021 May 26;17(12):4386–4400. doi: 10.1080/15548627.2021.1917130

Figure 1.

Figure 1.

TPD52 participates in the activation of CMA. (A) Anti-FLAG immunoprecipitates (IPs) coupled with mass spectrometry analysis (MS) to identify TPD52-interacting proteins in C4-2 cells. (B) IP-MS results were subjected to enrichment analysis. (C) The proteins were enriched in the “protein targeting” and “CMA” categories. (D-F) Endogenous TPD52 was increased after CMA activation. Immunoblot (IB) analysis of whole-cell lysates (WCLs) derived from C4-2 cells or TPD52-overexpressing PC3 cells treated with AR7 at the indicated concentration for 12 h (D), serum starved (S.S.) for 36 h and 48 h (E) or 6-AN at the indicated concentration for 24 h (F). (G) Endogenous TPD52 was increased after CMA activation in prostate tissues from BALB/c mice. IB analysis of WCLs derived from prostate tissues in the mice subjected to starvation or treated with a vehicle for 48 h. (H) Endogenous TPD52 was increased after CMA activation in the C4-2 xenograft tumors in the BALB/c nude mice. IB analysis of the WCLs derived from the C4-2 cell-implanted tumors in the BALB/c nude mice subjected to starvation or treated with a vehicle for 48 h