Figure 3.

TPD52-mediated CMA promotes PCa tumorigenesis through LAMP2A. (A–B) Representative images (A) and histogram of the quantified (B) LAMP2A cells stained for immunohistochemical analysis of PCa tissues paired with adjacent normal tissues (n = 45 per group). Scale bars: 200 μm (black); 50 μm (red). *P˂0.05. (C) IB analysis of the whole-cell lysates (WCLs) derived from the prostate tissues of the TRAMP and wild-type (WT) mice. (D–F) PC3 cells stably expressing scramble (Sc) or shLAMP2A in cells overexpressing vector (Vec) or TPD52 were subcutaneously injected into nude mice. (D) Statistical analysis of the tumor volume, which was measured every three days and plotted individually. (E) Subcutaneous xenograft tumors formed from different groups of PC3 cells were dissected. (F) Statistical analysis of the weight of the dissected xenograft tumors; n = 10 mice per experimental group, and the results are presented as the means ± S.D. *P˂0.05, **P˂0.01. (G) IB analysis of the WCLs derived from the subcutaneous xenograft tumors. (H) Representative images of immunohistochemical photomicrographs (left) and quantitative results (right) of MEF2D protein expression in the PCa tissues. Scale bars: 10 μm (red). The results indicate the mean ± S.D. **P˂0.01