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. 2021 Oct 27;22(10-12):587–597. doi: 10.1080/15384047.2021.1990631

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© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 6. — NSUN6 via 5mC regulates TATA binding protein complexes formation and the preinitiation complex formation

(a) Blot shows TATA binding protein (TBP) DNase foot printing in SgNT (lane 1) and SgNSUN6 (lane 2) and lane (3) SgNT treated with sarkosyl and lane (4) SgNSUN6 treated with sarkosyl. (b) Bar charts represent fold change of 5mC IP enrichment of TATA binding protein (TBP) normalized against GAPDH comparing SgNT (red) versus SgNSUN6 (green) total RNA. (c) Bar charts represent fold change of 5mC IP enrichment of GTF2A2 normalized against GAPDH comparing SgNT (red) versus SgNSUN6 (purple) total RNA (d) Bar charts represents fold change of 5mC IP enrichment of POLR2A normalized against GAPDH comparing SgNT (red) versus SgNSUN6 (black) total RNA. (e) Bar charts represent fold change of 5mC IP enrichment of GTF2B normalized against GAPDH comparing SgNT (red) versus SgNSUN6 (magenta) total RNA. (f) Bar charts represent fold change of 5mC IP enrichment of GTF2E normalized against GAPDH comparing SgNT (red) versus SgNSUN6 (gray) total RNA.