FIG. 1.

Targeted disruption of the Oct1 gene by homologous recombination. In structures of the wild-type and mutant alleles and the targeting construct, exons are indicated by closed boxes (exact positions and sizes of exons are not drawn to scale). In the targeting construct, exon 7 was replaced with an inverted (as indicated with an arrow) pgk-hygro cassette. Only relevant restriction sites are indicated: H, HindII; R, EcoRV; B, BamHI; N, NotI. For Southern analysis, 5′ and 3′ probes were used on HindII (5′) and EcoRV (3′) digested genomic DNA. Sizes of diagnostic restriction fragments for wild-type and targeted alleles are indicated by double-headed arrows (drawn to scale).