FIG. 1.

Ras blocks anoikis of RIE-1 cells. (A and B) RIE-1 cells stably expressing activated H-, K-, or N-Ras proteins were analyzed for viability by the MTS assay after being held in suspension for 15 h (A) or 12 h prior to assessing apoptosis by DNA laddering (B). (C) Cells stably infected with vector were incubated with vehicle or a broad-spectrum caspase inhibitor for 15 h in suspension prior to assessing apoptosis by DNA fragmentation ELISA. Data shown are representative of at least two independent experiments. Bars represent the standard deviations resulting from triplicate samples (A) or duplicate reading of the same sample (C).