FIG. 6.

Activated PI3K, Raf-1, or Rlf alone is not sufficient to block anoikis of ROSE 199 cells. ROSE 199 cells stably infected with pBabe-puro retrovirus vectors encoding oncogenic H-Ras or the plasma membrane-targeted and constitutively activated versions of the p110α subunit of PI3K, Raf-1, or HA-tagged Rlf were assayed for anoikis resistance after suspension for 4, 8, and 12 h by performing DNA fragmentation ELISA. Data shown are representative of two independent experiments. Bars represent standard deviations resulting from duplicate reading of the same sample.