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. 2001 Aug;21(16):5488–5499. doi: 10.1128/MCB.21.16.5488-5499.2001

FIG. 8.

FIG. 8

FIG. 8

Akt activation is not caused by Ras and is not involved in protection against anoikis. (A) H-Ras(12V)-expressing RIE-1 cells were held in suspension for 6 h in either the presence or the absence of serum and treated with either 10 μM LY294002 or vehicle. Cells were analyzed for activation of Akt by Western blot analysis with phospho-specific Akt antibodies. Blots were stripped and reprobed with anti-Akt antibodies to assess total Akt protein levels. (B) DNA laddering was evaluated in cells suspended for 4 and 12 h in the presence or absence of serum and treated with LY294002 or vehicle. (C) RIE-1 cells stably infected with the empty pZIP-NeoSV(x)1 expression vector or vector encoding H-Ras(61L), Raf-22W, or p110-CAAX were seeded in soft agar and assessed for colony formation after 10 days. The dark bar represents cells treated with 10 μM LY294002, and the light bar represents cells treated with vehicle.