Table 2.
Minimal residual disease detection by NGS.
| Specimen | Input DNA (ng) | Total reads | Reads | Clonal cell eq. per input DNA | Clonal cell eq. per 1000 ng | Tumor clone, interpretation | |||
|---|---|---|---|---|---|---|---|---|---|
| Clonal | LQ | (%) clonal | (%) LQ | ||||||
| Diagnostic BM | 100 | 365953 | 321793 | 5352 | 87.93 | 1.46 | 6013 | 60126 | Detected |
| After R-CHOP BM | 1000 | 743431 | 22865 | 26454 | 3.08 | 3.56 | 86 | 86 | Detected |
| After R-CHOP PB | 1000 | 177177 | 5487 | 70158 | 3.10 | 39.60 | 8 | 8 | Detected |
| Post Tx PB, TDNA, day 30 | 1000 | 220346 | 8 | 33874 | 0.0036 | 15.37 | 0.024 | 0.024 | Detected |
| After Tx PB, CD19+ DNA, day 30 | 500 | 2049647 | 22 | 35956 | 0.001 | 1.754 | 0.061 | 0.122 | Detected |
| After Tx PB, TDNA, day 60 | 250 | 173948 | 0 | 4099 | 0 | 2.28 | 0 | 0 | Not detected |
| After Tx PB, CD19+ DNA, day 60 | 250 | 301327 | 0 | 1652 | 0 | 0.55 | 0 | 0 | Not detected |
DNA input, total reads, % clonal or LQ reads, and clonal cell equivalents/1000 ng of DNA are shown. BM: bone marrow, PB: peripheral blood, TDNA: total DNA, CD19+: DNA from isolated CD19+ B cells, and Tx: allotransplant.