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. 2021 Dec 26;13(12):1928–1946. doi: 10.4252/wjsc.v13.i12.1928

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©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/

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Identification of nucleus pulposus-derived mesenchymal stem cells. A: Isolated primary cells presented with a long spindle shape and grew in flower formation; B: Nucleus pulposus-derived mesenchymal stem cells (NPMSCs) exhibited high fluorescent expression of CD73, CD90, and CD105, but low fluorescent expression of CD34 and CD45; C: NPMSCs was positive for Alizarin red, Oil Red O, and Alcian blue staining after induced differentiation. Scale bar = 50 μm. NPMSCs: Nucleus pulposus-derived mesenchymal stem cells.