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. 2021 Jul 29;36(1):68–79. doi: 10.1038/s41375-021-01346-7

Fig. 2. Igf2bp3 deletion delays leukemogenesis and reduces disease severity.

Fig. 2

a Leukemia-free survival of mice transplanted with control (Ctrl) or MLL-Af4-transduced HSPCs from WT or Igf2bp3 KO mice (Kaplan–Meier method with log-rank test; ****P < 0.0001). b Overall survival of mice transplanted with Ctrl or MLL-Af4-transduced HSPCs from WT or I3KO mice (n = 12 WT/Ctrl, n = 24 WT/MLL-Af4, n = 7 I3KO/Ctrl, n = 22 I3KO/MLL-Af4; Kaplan–Meier method with log-rank test; ****P < 0.0001). c Time course of WBC in the PB of mice transplanted with Ctrl or MLL-Af4-transduced HSPCs from WT or I3KO mice (data represented as means of three experiments; n = 4 Ctrl, n = 8 MLL-Af4 per experiment). d Spleen weights of mice transplanted with Ctrl or MLL-Af4-transduced HSPCs from WT or I3KO mice at 14 weeks (n = 4 Ctrl, n = 8 MLL-Af4; one-way ANOVA followed by Bonferroni’s multiple comparisons test; ****P < 0.0001). e H&E staining of liver and spleen of mice transplanted with mice transplanted with MLL-Af4-transduced HSPCs from WT or I3KO mice at 14 weeks. Scale bar: 100 μm; CV central vein; W white pulp; R red pulp; Leu leukemia; arrows showing infiltration. f Quantitation of CD11b+Ki67+ cells in the spleen at 14 weeks post transplantation (n = 4 Ctrl, n = 8 MLL-Af4; one-way ANOVA followed by Bonferroni’s multiple comparisons test; *P < 0.05). g (Left) Number of CD11b+ in the SP of recipient mice that received Ctrl or MLL-Af4-transduced HSPCs from WT or I3KO mice at 14 weeks (one-way ANOVA followed by Bonferroni’s multiple comparisons test; **P < 0.01). (Right) Corresponding representative FACS plots showing CD11b+ and B220+ cells in the SP.