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. 2021 Jun 25;36(1):236–247. doi: 10.1038/s41375-021-01327-w

Fig. 4. EPAG stimulates human CD45+ CD61+ MDS and not residual healthy megakaryopoiesis in patient-derived xenografts.

Fig. 4

A FACS plots showing sorting gates and percentage (top left corner) of human CD45+ (hCD45+) and hCD45+ hCD61+ cells in the mononuclear cells of patient P04 and pooled bone marrow samples from n = 3 CTRL and n = 3 EPAG patient-derived xenografts (PDX) at endpoint. B Sanger sequencing results of PCR-amplified DNA from sorted hCD45+ and hCD45+ hCD61+ cells of patient P04, as well as CTRL and EPAG PDX for the patient’s MDS-specific mutation SRSF2P95H. Red arrow indicates the side of mutation where cytosine (C, blue) was replaced by adenine (A, green). Results for the remaining patient–individual mutations are shown in Supplementary Fig. S6A–C. SSC-A, side scatter area.