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. 2021 Dec 31;7(1):132–144. doi: 10.1038/s41564-021-01023-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — The Fisher’s alpha diversity index (a), the Simpson’s diversity index (b), the Chao1 estimated richness (c), and the observed richness (d) did differ significantly across the 11 3-month age groups as analysed by the non-parametric Kruskal-Wallis test. Data were tested for normality using the Anderson-Darling and the D’Agostino & Pearson test in Prism 9 for macOS and were non-normally distributed. Data were considered to be statistically significant with a confidence level of 95%. Data were plotted using the Scatter dot plot function in Prism 9 for macOS with a line drawn at the mean with error bars extending to the standard deviation. In this overall general presentation of the alpha diversity indexes, all 1389 samples across the three sampling timepoints were combined. The same analysis was performed for the three different individual sampling timepoints Day 1, Day 15, and Day 85 which is shown in Extended Data Fig. 3.