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. 2021 Dec 30;62(15):31. doi: 10.1167/iovs.62.15.31

Figure 3.

Figure 3.

Study design and scRNA-seq analysis of immune cells. (A) Experimental design for sc-RNA seq. Samples of CDLNs were collected from normal mice, EAU mice, and IL38-treated EAU mice. Each sample included three mice. The gene expression data of samples were obtained via scRNA sequencing for subsequent single-cell analysis. (B) The t-SNE clustering of CDLN cells. (C, D) Pie charts and Bar charts show proportions of each cell type across three groups derived from scRNA-seq data. (E) Volcano plot showing up- and down-regulated DEGs of CDLN cells in the EAU/Normal and IL-38/EAU comparison groups. Red and blue dots indicate up- and down-regulated DEGs, respectively. (F) GO analysis of downregulated DEGs of CDLN cells in the IL-38/EAU comparison groups. The q value was calculated using the Benjamini-Hochberg procedure. (G) Rose diagrams showing the numbers of up- and down-regulated DEGs of CDLN cells in the EAU/Normal and IL-38/EAU comparison groups. (H) GO analysis of downregulated DEGs of T cells in the IL-38/EAU comparison groups. q value was calculated using the Benjamini-Hochberg procedure.