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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: FEBS J. 2021 Jul 26;289(1):215–230. doi: 10.1111/febs.16122

Figure 3. PCPG LUVs modulate the inhibitory efficacy of EGCG and resveratrol during seeded and unseeded αS aggregation.

Figure 3.

Fibril formation of (A) unseeded αS (150 μM) and (B) seeded αS (50 μM with 2.5% w/w seed fibrils) in the presence of EGCG (50 μM) and resveratrol (50 μM) was monitored through ThT fluorescence assays in the presence and absence of PCPG LUVs (1:30 αS:LUV). The extent of fibril inhibition afforded by EGCG and resveratrol was calculated by comparing the average ThT fluorescence maximum in the final hour of the assay across each treatment for (C) unseeded and (D) seeded assays, and the time taken to reach half maximum ThT fluorescence (t50) was also compared (E and F for seeded and unseeded assays respectively). Data are reported as mean ± SEM (n = 3), *p<0.05.