Figure 4.

Accumulation of ShTPS12- and ShSBS-derived sesquiterpenes in tomato leaves expressing the multicistronic constructs. Terpenes were extracted from tomato leaves infiltrated with Agrobacterium carrying the pC5-FTG construct (A), the pC5-zFSG construct (B), or the empty pMCS vector (EV) (C) and were analyzed by GC–MS (total ion chromatograms are shown). ShTPS12-derived sesquiterpenes: 1, β-caryophyllene; 2, α-humulene. ShSBS-derived sesquiterpenes: 3, (−)-endo-α-bergamotene; 4, (+)-α-santalene; 5, (−)-exo-α-bergamotene; 6, (−)-epi-β-santalene; and 7, (+)-endo-β-bergamotene. The total amounts (pmol/cm² leaf area) of ShTPS12-derived (D) and ShSBS-derived (E) sesquiterpenes were determined in tomato leaves at different time points after the Agrobacterium infiltration. Data are means ± SEM (n = 3).