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. 2021 Dec 22;11:778132. doi: 10.3389/fonc.2021.778132

Figure 1.

Figure 1

Expression of N6-methyladenosine methyltransferase METTL3 and m6A level is decreased in TNBC tissues and cell lines. (A) The mRNA level of METTL3 in TNBC tissues and paired normal tissues were detected by real-time PCR (n=30). (B) The protein level of METTL3 in TNBC tissues and paired normal tissues were detected by Western Blot (n=6). (C) The mRNA level of METTL3 in 10 breast cell lines, including 9 breast cancer cell lines (MDA-MB-231, MDA-MB-469, MCF-7, BT-20, BT-483, BT-474, BT-549, T47D and SKBR3) and one HME cell lines (MCF-10A) were detected by real-time PCR. (D) The protein level of METTL3 in 10 breast cell lines were confirmed by Western Blot. (E) The global m6A RNA level in TNBC tissues and paired normal tissues were detected by Colorimetric m6A RNA Methylation Assay Kit (n=30). (F) The global m6A RNA level in TNBC tissues and paired normal tissues were confirmed by Dot Blot (n=6). (G) The global m6A RNA level in 10 breast cell lines were detected by Colorimetric m6A RNA Methylation Assay Kit. (H) The global m6A RNA level in 10 breast cell lines were confirmed by Dot Blot. **P < 0.01 and ***P < 0.001 versus indicated group.