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. 2021 Dec 22;11:778132. doi: 10.3389/fonc.2021.778132

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Copyright © 2021 Ruan, Gu, Xia, Gong, Zhou, Chen and Xiong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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METTL3 is directly targeted by miR-34c-3p. (A) The putative bind site between METTL3 3’UTR and miR-34c-3p was predicted by Target Scan tool. (B) Two dual-luciferase reporter vectors containing wild-type METTL3 3’-UTR (METTL3 3’-UTR-WT) or site mutant METTL3 3’-UTR (METTL3 3’-UTR-Mut) were constructed. (C) Dual-luciferase assay was used to evaluate the targeting of miR-34c-3p to wild-type METTL3 3’-UTR. (D) The level of METTL3 mRNA in normal or miR-34c-3p over-expressed TNBC cells was detected by real-time PCR. (E) The level of METTL3 protein in normal or miR-34c-3p over-expressed TNBC cells was detected by Western Blot. *P < 0.05 versus indicated group.