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. 2021 Dec 22;11:756011. doi: 10.3389/fonc.2021.756011

Figure 2.

Figure 2

ADSC promotes EMT by activating the TGF-β signaling pathway in OC cells. (A, B) Western blot analysis of EMT markers in both OVCAR3 (A) and OVCAR8 (B) cells following treatment with ADSC-CM for different time durations (0, 24, and 48 h). Right, the quantification analysis of the blots. (C, D) Western blot analysis of the p-SMAD2 and SMAD2/3 expression in both OVCAR3 (C) and OVCAR8 (D) cells following treatment with ADSC-CM for different time durations. Right, the quantification analysis of the blots. (E, F) Luciferase activity in OVCAR3 (E) and OVCAR8 (F) cells following ADSC-CM treatment for 24 h, then 6 ng/ml TGF-β treatment for 12 h. (G) ELISA analysis of ADSC-CM collected at different time points to detect the concentrations of TGF-β1. Data represent the mean ± SD of three independent experiments. ** p < 0.01, *** p < 0.001 compared with the control group.