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. 2021 Dec 22;12:782989. doi: 10.3389/fimmu.2021.782989

Figure 6.

Figure 6

Menthol alleviates the inflammatory response of BMECs through the AMPK signaling pathway. BMECs were pretreated with 200 μM menthol and CC for 1h and treated with 5 μg/ml LPS for 12 h. (A) Protein expression results of INOS and COX-2 detected by western blotting(n≥3). (B, C) Bar graphs indicate the quantitative results of the corresponding protein bands. β-actin was used as an internal reference protein to homogenize the protein results. (D–F) Results of qRT-PCR for mRNA levels of IL-1β, IL-6, and TNF-α. β-actin was used as an internal reference for normalization of mRNA expression(n≥3). (G) Detection of Nrf-2 entry by immunofluorescence staining of the nucleus (blue) and Nrf-2 (green). (H) Protein expression results of N-Nrf-2 and Histone H3 detected by western blotting(n≥3). (I) Bar graphs indicate the quantitative results of the corresponding protein bands.Histone H3 was used as an internal reference protein to homogenize the protein results. The values are presented as the means ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001).