Skip to main content
. 2021 Dec 22;12:782989. doi: 10.3389/fimmu.2021.782989

Figure 8.

Figure 8

Menthol alleviates the inflammatory response through the Nrf-2/HO-1 signaling axis. The BMECs were first pretreated with 200 μM menthol and ML385 for 1 h. LPS at 5 μg/ml was added for 12 h. (A) Protein expression results of INOS and COX-2 detected by western blotting (n≥3). (B, C) The bar graphs indicate the quantitative results of the corresponding protein bands. The protein results were homogenized using β-actin as an internal reference protein. (D–F) Results of qRT-PCR to detect the mRNA levels of IL-1Bβ, IL-6, and TNF-α (n≥3). The mRNA expression of β-actin was used to normalize the mRNA expression of the above proteins. (G) Immunofluorescence staining to detect the entry of Nrf-2 into the nucleus, nucleus (blue), and Nrf-2 (green). (H) Protein expression results of N-Nrf-2 and Histone H3 detected by western blotting(n≥3). (I) Bar graphs indicate the quantitative results of the corresponding protein bands.Histone H3 was used as an internal reference protein to homogenize the protein results. The values are presented as the means ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001).