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. 2021 Mar 27;11(12):4045–4054. doi: 10.1016/j.apsb.2021.03.036

Figure 3.

Figure 3

DFO could trigger significantly the treatment of baicalin in bladder cancer cells. (A) and (B) The cell death was observed by flow cytometer with the treatment of baicalin with or without DFO, mean ± SD, n = 3, ∗∗P < 0.01. (C) 5637 and KU-19-19 were treated with baicalin along with or without DFO for 24 h, then intracellular chelate iron was analyzed, mean ± SD, n = 3; ∗P < 0.05, ∗∗P < 0.01. (D) The reactive oxygen species (ROS) level was analyzed by a flow cytometer, mean ± SD, n = 3; ∗∗P < 0.01. (E) Mito-Tracker Green staining was made to detect mitochondria damage. Scale: 100 μm. (F) The expression of FTH1, HO-1, transferrin in bladder cancer cells was detected after the treatment with baicalin with or without DFO for 24 h by Western blotting. (G) qRT-PCR was performed to detect the mRNA expression of transferrin, mean ± SD, n = 3; ∗∗P < 0.01.