Figure 1.

Effects of BEZ235, copanlisib and rapamycin on proliferation of endothelial and osteoblastic niche cells. HMEC-1 cells (A), CAL-72 cells (B), Mono Mac 6 cells (C), THP-1 cells (D), HUVEC cells (E), primary osteoblasts (F) and primary macrophages isolated from heathy donors (G) were incubated in control medium (Co) or in medium containing various concentrations of BEZ235 (0.005-5 μM), copanlisib (0.01-5 μM) and rapamycin (0.01-5 μM) at 37°C for 48 hours. Thereafter, ³H-thymidine uptake was measured. Results show ³H-thymidine uptake as percentage of control (=100%, Co) and represent the mean ± SD of three independent experiments. Asterisk (*): P<0.05 compared to control.