Figure 4.

Effects of combinations of BEZ235 or copanlisib with nilotinib or BEZ235 or copanlisib with ponatinib on survival of endothelial and osteoblastic niche cells. HMEC-1 cells (A) and CAL-72 cells (B) were incubated in control medium (0), in medium containing BEZ235, copanlisib, nilotinib or ponatinib, alone or in combinations (BEZ235+nilotinib, copanlisib+nilotinib, BEZ235+ponatinib, copanlisib+ponatinib) as indicated at 37°C for 48 hours. Then, cells were examined by flow cytometry to determine the percentage of Annexin-V-positive cells. Results represent the mean ± SD of three independent experiments. Asterisk (*): P<0.05 compared to control.