Figure 2.

Dose-response curves of the drugs, U0126, and LY294002, and their combination. Effects of U0126 and LY294002 on proliferation in B16F10 mouse melanoma cells (A-C) was done by MTT assay after 72 h of treatment. Data shown are mean ± SEM of three replicate wells. The B16F10 cells were treated with 5-60 µM of U0126 (A) and LY294002 (B). Drug combination (C) was done in the concentration range 5-50 µM, i.e., 5 µM U0126 + 5 µM Ly294002 to 50 µM U0126 + 50 µM Ly294002. A graph was plotted with concentration of the inhibitor (X-axis) vs cell death % (Y-axis) and the IC₅₀ values were calculated using the formula y = b + ax. The IC₅₀ value is the concentration of the drug which results in 50% cell death.