Figure 10.

Western blot analysis of the valproic acid-treated B16F10C and B16F10R cells. Control (B16F10C) and drug-resistant (B16F10R) cells were treated with VPA (2 mM) for 24 h and cells were lysed using lysis buffer. Apoptotic proteins-cleaved Caspase-3 (Cl_Caspase 3), cleaved Caspase-9 (Cl_Caspase) and pyruvate dehydrogenase complex (PDH) were detected by western blots in both untreated (B16F10C and B16F10R) and VPA treated cells (B16F10C+VPA and B16F10R+VPA). (A) Western blot of the different proteins. β-actin served as loading control. The comparison of the quantified proteins Cl_Caspase 3, Cl_Caspase 9 and PDH are shown (B-D), respectively. *indicates the statistically significant difference (P<0.05).