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. 2021 Dec 15;11(12):5992–6003.

Figure 3.

Figure 3

Jagged-1/2 could stimulate tumor-associated stromal cells (HSF) to secrete IL-6 by up-regulating the notch signaling pathway. (A, B) HSF cells were collected after treated with 4 μg/ml Jagged-1 or 5 μg/ml Jagged-2 for 24 hours, for measuring mRNA expression levels of IL-6 (A), Notch-1/2/3 (B) by RT-qPCR. (C, D) HSF cells were cultured with/without wild type, Jagged-1 or Jagged-2 knockdown PaTu-8988 (C) or PANC-1 cells (D), then culture media were collected and subjected to IL-6 quantification by ELISA after 24 hours co-incubation. (E) HSF cells were pretreated with Nirogacestat or DMSO for half an hour, then the HSF cells were co-cultured with or without PaTu-8988/PANC-1 cells, after that, culture media were collected and subjected to IL-6 quantification by ELISA after 24 hours co-incubation. (*P<0.05, **P<0.01, ***P<0.001 versus control group).