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. 2021 Dec 15;11(12):5992–6003.

Figure 4.

Figure 4

IL-6 could upregulate the Jagged-1/2 expression level in pancreatic cancer cells. (A-D) The PaTu-8988 (A, B) and PANC-1 (C, D) were treated with recombinant human IL-6 (rhIL-6, 100 ng/ml) for different times, and collected the cells for analyzing Jagged-1/2 expression level by RT-qPCR or immunoblotting. (E-H) PaTu-8988 or PANC-1 were co-culture with (or without) wild type or IL-6 knockdown HSF, or added IL-6 antibody in the co-culture medium, then the Patu-8988 or PANC-1 cells in the upper chambers were collected for analyzing Jagged-1 and Jagged-2 expression level by RT-qPCR or immunoblotting. (*P<0.05, **P<0.01, ***P<0.001 versus the control group).