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. 2021 Dec 15;11(12):5992–6003.

Figure 5.

Figure 5

STAT3 and NF-κB signaling were involved in IL-6-promoted Jagged-1/2 expression in pancreatic cancer cells and Jagged1/2-enhanced HSF production of IL-6, respectively. A. The PaTu-8988 cells were pretreated with (or with) DMSO, U0126, MK2206 or STATTIC for half an hour, then the cells were challenged with rhIL-6 for 12 hours, after that, the cell were subjected to immunoblotting with corresponding antibodies. B. PaTu-8988 were transfected with control shRNA or STAT3 shRNA lentiviruses for 48 hours, then the cells were challenged with rhIL-6 for 12 hours, after that, the cell were subjected to immunoblotting with corresponding antibodies. C. HSF cells were pretreated with (or without) PDTC for half an hour, then the cells were treated with 4 μg/ml Jagged-1 or 5 μg/ml Jagged-2 for 24 hours, after that, the cell were collected for analyzing IL-6 mRNA level by RT-qPCR. D. HSF cells were transfected with control shRNA or STAT3 shRNA lentiviruses for 48 hours, then the HSF cell challenged with 4 μg/ml Jagged-1 or 5 μg/ml Jagged-2 for 24 hours, after that, the cells were collected for analyzing IL-6 mRNA level by RT-qPCR. (*P<0.05, **P<0.01, ***P<0.001 versus the control group; Different alphabets denote a significant difference at P<0.05).