Skip to main content
. 2021 Jun 25;11(12):4008–4019. doi: 10.1016/j.apsb.2021.04.003

Figure 3.

Figure 3

JOSD2 interacts with YAP/TAZ. (A) Representative images of immunofluorescence with JOSD2 in green, YAP in red and DAPI in blue shows high co-localization of YAP and JOSD2 in CCLP-1 cells. The pearson's and overlap coefficient ratio were determined by Image J. The results represent as the mean ± SD, n = 100. (B) and (C) Interaction between exogenous JOSD2 and YAP/TAZ. HA-tagged JOSD2 and Flag-tagged YAP or TAZ plasmids were co-transfected into 293T, followed by incubation of cellular extracts and anti-HA magnetic beads or anti-DYKDDDDK (anti-Flag) IP resin. Immunoblotting was performed with indicated antibodies. (D) Interaction between endogenous JOSD2 and exogenous YAP/TAZ. Exogenous Flag-tagged YAP or TAZ was transfected into 293T, then the cell lysate was prepared for Co-IP with anti-DYKDDDDK IP resin and examined by immunoblotting. (E) Endogenous interaction of YAP/TAZ with JOSD2 was determined by Co-IP analyses using antibodies against YAP or TAZ, and followed by the immunoblotting with anti-JOSD2 antibody.