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. 2021 Jun 25;11(12):4008–4019. doi: 10.1016/j.apsb.2021.04.003

Figure 5.

Figure 5

JOSD2 regulates CCA cell proliferation in vivo. (A, B) JOSD2 depletion arrests the growth of HuCCT-1 xenograft tumors. The HuCCT-1 xenograft bearing mouse was passaged for intratumor injection of shJOSD2 virus every two days. RTV is expressed as mean ± SEM, n = 8/group; ∗∗∗P < 0.001. (C) Knockdown of JOSD2 decreases tumor weight as present. n = 8/group; ∗∗P < 0.01. (D) and (E) The knockdown efficiency was confirmed by immunoblotting and the intratumor YAP/TAZ protein levels were decreased as quantified by Image J. ∗∗P < 0.01, ∗P < 0.05. (F) and (G) JOSD2 depletion arrests the growth of PDX. RTV is expressed as mean ± SEM, n = 8/group; ∗∗∗P < 0.001. (H) Knockdown of JOSD2 decreases PDX tumor weight as present; ∗∗P < 0.01. (I) and (J) The knockdown efficiency of JOSD2 and intratumor YAP/TAZ protein levels was confirmed by immunoblotting and quantified by Image J; ∗∗∗P < 0.001.