FIG. 5.

The Bcl-2 apoptotic pathway is involved in apoptotic response of induced U937T-A/E cells. (A) RPA analyses showing the reduced expression of Bcl-2 mRNA. Total RNA (10 μg) was prepared from U937T and derived clones cultured for 72 h after withdrawal of tetracycline and hybridized with α-³²P-labeled in vitro-transcribed probes from the Bcl-2 family (only Bcl-2 and Bax are represented along with L32 and GAPDH [glyceraldehyde-3-phosphate dehydrogenase] as the internal control) and resolved on a denaturing polyacrylamide gel. (B) Western blot analyses showing the expression of Bcl-2 proteins in U937T cells and U937T-A/E pool 18 and 48 cells following tetracycline withdrawal for 72 h. Ponceau red staining is shown to indicate the relative amount of protein in each lane.